# Analytical Chemistry – Fluorometric method

(See attached file for full problem description)

23. In developing a fluorometric method, you measure a standard Analyte at 3.00 ppm (w/w) in the matrix at three different pH values: 2,5,8. You then compare the effects of internal calibration by adding 2.00ppm Analyte to your matrix vs external calibration via pure standards and obtain the following data

pH= 2 Slope=2607
Intercept = 3466
Measured signal for 3.00ppm standard =12781
Signal after 2.00ppm spike =15611

pH= 5 Slope=2651
Intercept = 1232
Measured signal for 3.00ppm standard =9215
Signal after 2.00ppm spike =14503

pH= 8 Slope=434
Intercept = 1156
Measured signal for 3.00ppm standard =2205
Signal after 2.00ppm spike =2920
A) Derive the equations needed to solve the concentration from i) the straight line equation, y=mx+b and the measured signal ‘S’ for the external and ii) the data points (0,Ssignal), and (Cspike, Ssignal+spike).
B) Determine which pH condition has (I) little influence from the matrix, (II) decreased signal because of the matrix, and (III) false enhancement of signal from the matrix. Be sure to subtract the background signal!

Calculate the price
Pages (550 words)
\$0.00
*Price with a welcome 15% discount applied.
Pro tip: If you want to save more money and pay the lowest price, you need to set a more extended deadline.
We know how difficult it is to be a student these days. That's why our prices are one of the most affordable on the market, and there are no hidden fees.

Instead, we offer bonuses, discounts, and free services to make your experience outstanding.
How it works
Receive a 100% original paper that will pass Turnitin from a top essay writing service
step 1
Fill out the order form and provide paper details. You can even attach screenshots or add additional instructions later. If something is not clear or missing, the writer will contact you for clarification.
Pro service tips
How to get the most out of your experience with AssignmentHelpWeb
One writer throughout the entire course
If you like the writer, you can hire them again. Just copy & paste their ID on the order form ("Preferred Writer's ID" field). This way, your vocabulary will be uniform, and the writer will be aware of your needs.
The same paper from different writers
You can order essay or any other work from two different writers to choose the best one or give another version to a friend. This can be done through the add-on "Same paper from another writer."
Copy of sources used by the writer
Our college essay writers work with ScienceDirect and other databases. They can send you articles or materials used in PDF or through screenshots. Just tick the "Copy of sources" field on the order form.
Testimonials
See why 20k+ students have chosen us as their sole writing assistance provider
Check out the latest reviews and opinions submitted by real customers worldwide and make an informed decision.
Thank you!
Customer 452451, November 27th, 2022
Excellent service - thank you!
Customer 452469, February 20th, 2023
Excellent timely work
Customer 452451, April 19th, 2023
Anthropology
Excellent services will definitely come back
Customer 452441, September 23rd, 2022
Job well done. Finish paper faster than expected. Thank you!
Customer 452451, October 3rd, 2022
Nursing
The paper was EXCELLENT. Thank you
Customer 452449, September 23rd, 2022
Theology
Job well done and completed in a timely fashioned!
Customer 452451, November 18th, 2022
Anthropology
excellent loved the services
Customer 452443, September 23rd, 2022
Psychology
Thanks a lot the paper was excellent
Customer 452453, October 26th, 2022
English 101
Very good job. I actually got an A
Customer 452443, September 25th, 2022
Architecture, Building and Planning
The assignment was well written and the paper was delivered on time. I really enjoyed your services.
Customer 452441, September 23rd, 2022
11,595
Customer reviews in total
96%
Current satisfaction rate
3 pages
Average paper length
37%
Customers referred by a friend