Determine the Maximum Binding Constant for a Protein Complex
Plot the following data using a Sacatchard type plot. The change in fluorscence is proportional to binding.
A) Determine the max binding constant for the protein complex.
Realtive Fluorescence (Response) [Protein] nM (concentration of added protein)
5.70E+04 | 0
5.73E+04 | 0.1
5.78E+04 | 0.3
5.89E+04 | 1
6.03E+04 | 3
6.13E+04 | 10
6.17E+04 | 30
6.18E+04 | 50