Translocation of proteins
A. Explain the experimental results that are seen in the absence of microsomes (Figures 8-10, lanes 1 to 4).
B. Using the three criteria outlined in the problem decide whether the experimental results in the presence of microsomes (lanes 5 to 8) indicate that the protein is translocated across microsomal membranes. Explain the migration of the proteins in lanes 5, 6 and 8.
C. Is the protein anchored in the membrane, or is it translocated all the way through the membrane?
These questions are posed.